Canine renal failure syndrome in three dogs

Three dead dogs were brought to the College of Veterinary Medicine, Kyungpook National University for study. Clinically, all the dogs showed emaciation, anorexia, depression, hemorrhagic vomiting and diarrhea for 7~10 days before death. All the clinical signs were first noted for about one month after feeding the dogs with commercial diets. At necropsy, all 3 dogs had severe renal damage with the same green-yellowish colored nephroliths in the renal pelvis. They also showed systemic hemorrhage and calcification of several organs, which might have been induced by uremia. Microscopically, necrosis, calcification and calculi were detected in the renal tubules, and especially in the proximal convoluted tubules and collecting ducts of the kidney. These findings were supportive of a mycotoxic effect, and especially on their kidneys. However, the precise cause of the toxic effect in these cases of canine renal failure could not be determined.     

Multiple intestinal lymphomatous polyposis in a Jindo dog

A male, 5-year-old Jindo dog underwent enterectomy and enteroanastomosis due to ileus of the intestine at a local veterinary hospital. Grossly, the excised intestine showed markedly thickened multinodular masses in the serosal layer of the upper part, and soft-to-firm, cream-colored neoplastic masses that displayed extensive nodular mucosal protuberances into the lumen. The neoplastic masses were filled with large round cells that were ovoid in shape and they had pale and/or hyperchromatic nuclei. The neoplastic cells had mainly infiltrated into the mucosal and submucosal layers, and they had diffusely invaded the muscular and serosal layers. Therefore, the diagnosis of canine multiple intestinal malignant lymphomatous polyposis was made based on the gross and histopathological findings. The origin of these tumor cells was determined to be B-cells since they were positive for anti-CD20.     

转基因番茄F2代的贮藏特性研究

以F1′、F2、F3、NR四个转基因番茄基因型为实验材料,研究了转反义乙烯基因F2代番茄果实的贮藏特性,据此对转基因番茄各基因型的贮藏特性作综合分析评价。结果表明,F3基因型果实的失重率、腐烂率最低,果柄拉力最大,外观形态最好,贮藏特性最好;其次是F1′和NR基因型;而F2基因型最差。     

基于颠茄发根的外源基因表达系统的建立

 用携带植物高效表达载体pCAMBIA1304 ⁺ “解除武装”的重组C58C1工程菌转化颠茄无菌苗真叶, 发根诱导率达100%。PCR检测证实发根型质粒pRiA4和表达型质粒pCAMBIA1304 ⁺均能整合到颠茄发根基因组内, 共转化率达30%。建立了基于颠茄发根的高效外源基因表达系统, 为将托品烷类生物碱东莨菪碱的生物合成关键酶基因导入颠茄, 实现其次生代谢工程及开展分子育种奠定了基础。     

脱落酸、水杨酸和草酸诱导苹果叶片脯氨酸积累的效应

 以3年生盆栽红富士(Malus domestica Borkh. ) /湖北海棠〔Malus hupehensis (Pamp) Rehd. 〕为试材, 研究了脱落酸、水杨酸和草酸预处理对叶片脯氨酸的诱导作用, 结果表明, 预处理第2天, 自然干旱效果还不明显时, 水杨酸预处理就使叶片脯氨酸含量提高了7.87%; 自然干旱、脱落酸、水杨酸和草酸预处理至第9天, 叶片脯氨酸含量较对照分别提高了96.30%、110.67%、135.12%和115.96%; 预处理9 d后停止供水至第5天时, 叶片瞬时水分利用效率分别提高了286.77%、75.76%、79.04%和100.44% ,脯氨酸含量也明显提高; 停止供水至第10天时, 脯氨酸含量分别提高了5.33%、262.76%、92.32%和0。综合比较显示, 自然干旱、脱落酸、水杨酸和草酸预处理都可以通过提高叶片脯氨酸含量和水分利用效率而增强苹果的抗旱性, 其中脱落酸的效果最好, 其次是水杨酸, 草酸效果持续时间比较短。     

石斛属植物的研究进展

 摘　要: 综述了近10年来石斛属植物在系统与分类学、细胞学、园艺学及育种学等多学科的研究成果, 并对未来我国石斛属植物的研究发展进行了展望。     

EHEG O157△ler/stx(pBR322∷stx 1/2B∷eae)的构建与生物学特性分析

为了获得安全性好,对出血性大肠杆菌具有良好免疫保护作用的疫苗株,本实验以构建的O157△ler/stx基因缺失突变株为载体,将决定特异性粘附的eae全基因插入到质粒pBR322∷stx1/2B中,构建了基因工程菌O157△ler/stx(pBR322∷stx1/2B∷eae),对该基因工程菌进行生物学特性分析,实验结果表明,该菌正常培养可表达eae;具有粘附HEp-2细胞的特性;该菌培养上清对Vero细胞没有毒性作用;给小鼠口服接种该工程菌后可在肠道中定居至少10 d;14日龄小鼠口服活菌量为5×109CFU/只,小鼠生长正常,没有出现任何临床症状;该菌通过口服免疫小鼠可以诱导肠道粘膜免疫应答和体液免疫应答.这些结果表明,该工程菌安全性好,免疫原性强,是有价值的预防EHEC O157感染的基因工程疫苗候选株.     

黄皮洋葱新品种连葱4号的选育

连葱4号是以连引洋葱1号为母本、富永中生为父本配组杂交后,经多代系统选择育成.具有优质、高产、抗病等优点.平均每667 m2产量5000～6 500kg,适于长江流域地区种植.     

植物光自养微繁技术研究进展

 光自养微繁技术是一种全新的植物组织培养技术, 从提出该理论至今, 许多研究者已在光自养微繁方面做了大量的尝试, 使这一新技术逐步完善。本文对光自养微繁的研究进展进行了综述, 介绍了光自养微繁研究现状、研究热点、在种苗工厂化生产中的应用, 并提出光自养微繁技术研究动向。     

外源氯对番茄幼苗生长及养分吸收、利用的影响

 研究了不同水平外源氯处理对番茄(Lycopersicon esculentum L. ) 干物质积累、叶绿素含量、Cl^- 、NO₃^- 、氮、磷、钾、钙和镁含量的影响。结果表明: 一定范围( 6.25～100 nmol·L ^{- 1} ) 的外源氯处理, 不降低番茄生物量甚至促进生物量, 并可明显提高番茄幼苗对钾、镁、磷的吸收和氮素利用效率, 而200、300 mmol·L ^{- 1} Cl^- 处理的幼苗干物质积累极显著下降。